Single-cell analysis by use of ICP-MS
| Autor: | Larissa Mueller, Konrad Loehr, Norbert Jakubowski, Gunda Koellensperger, Sarah Theiner |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Materials science Calibration and validation Laser ablation 010401 analytical chemistry Nanotechnology Cellular level Mass spectrometry 01 natural sciences 0104 chemical sciences Analytical Chemistry 03 medical and health sciences Single-cell analysis Mass cytometry Instrumentation (computer programming) Inductively coupled plasma mass spectrometry Spectroscopy 030304 developmental biology |
| Zdroj: | Journal of Analytical Atomic Spectrometry. 35:1784-1813 |
| ISSN: | 1364-5544 0267-9477 |
| DOI: | 10.1039/d0ja00194e |
| Popis: | This tutorial review article is highlighting the fundamentals, instrumentation, and most recent trends of single-cell analysis by use of inductively coupled plasma-mass spectrometry (ICP-MS). It is shown that metals and hetero-elements being intrinsically present in cells, taken up by cells (for instance engineered metallic nanoparticles) or binding to a cell can be detected qualitatively by existing ICP-MS technologies on a single cell level. Adding a quantitative dimension to single-cell analysis by (laser ablation-) ICP-MS requires dedicated calibration and validation strategies, which are currently being established and are being critically discussed. In a tutorial part, the ICP-MS instruments, the measurement conditions, and the sample introduction and preparation techniques are introduced. The application section focuses on the state-of-the-art of single-cell analysis in suspension, using laser ablation or (imaging) mass cytometry. Finally, future trends are critically assessed. |
| Databáze: | OpenAIRE |
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