Characterization of trophic changes and a functional oxidative pentose phosphate pathway in Synechocystis sp. PCC 6803
| Autor: | Michael L. Summers, Tove Jansén, Dominic Kurian, Steve York, Pirkko Mäenpää, Wuttinun Raksajit |
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| Rok vydání: | 2009 |
| Předmět: |
Cyanobacteria
chemistry.chemical_classification Inorganic pyrophosphatase biology Physiology Permease Dehydrogenase Plant Science Pentose phosphate pathway Phosphate biology.organism_classification chemistry.chemical_compound Enzyme chemistry Biochemistry Glucose-6-phosphate dehydrogenase Agronomy and Crop Science |
| Zdroj: | Acta Physiologiae Plantarum. 32:511-518 |
| ISSN: | 1861-1664 0137-5881 |
| DOI: | 10.1007/s11738-009-0428-7 |
| Popis: | Cyanobacteria have a tremendous activity to adapt to environmental changes of their growth conditions. In this study, Synechocystis sp. PCC 6803 was used as a model organism to focus on the alternatives of cyanobacterial energy metabolism. Glucose oxidation in Synechocystis sp. PCC6803 was studied by inactivation of slr1843, encoding glucose-6-phosphate dehydrogenase (G6PDH), the first enzyme of the oxidative pentose phosphate pathway (OPPP). The resulting zwf strain was not capable of glucose supported heterotrophic growth. Growth under autotrophy and under mixotrophy was similar to that of the wild-type strain, even though oxygen evolution and uptake rates of the mutant were decreased in the presence of glucose. The organic acids citrate and succinate supported photoheterotrophic growth of both WT and zwf. Proteome analysis of soluble and membrane fractions allowed identification of four growth condition-dependent proteins, pentose-5-phosphate 3-epimerase (slr1622), inorganic pyrophosphatase (sll0807), hypothetical protein (slr2032) and ammonium/methylammonium permease (sll0108) revealing details of maintenance of the cellular carbon/nitrogen/phosphate balance under different modes of growth. |
| Databáze: | OpenAIRE |
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