Sulfhydryl reactive microspheres for the efficient delivery of thiolated bioactive cargoes

Autor:	Juan Manuel Cardenas-Maestre, Sonia Panadero-Fajardo, Rosario M. Sánchez-Martín, Ana M. Pérez-López
Rok vydání:	2011
Předmět:	Materials science Fluorophore medicine.diagnostic_test Nanoparticle Nanotechnology General Chemistry Safe delivery Flow cytometry Green fluorescent protein Microsphere chemistry.chemical_compound chemistry Materials Chemistry medicine Biophysics Gene silencing Conjugate
Zdroj:	Journal of Materials Chemistry. 21:12735
ISSN:	1364-5501 0959-9428
Popis:	A novel strategy for the efficient and safe delivery of thiolated therapeutic cargoes into mammalian cells has been developed. For this purpose, polymeric microspheres have been successfully functionalised to become a sulfhydryl reactive delivery system. SEM, DLS and zeta potential analysis were used to characterise these modified nanoparticles. The strategy has been validated by successfully conjugating a thiolated fluorophore and siRNA which silence GFP expression. The high efficiency of siRNA–microsphere conjugates as gene silencing devices has been demonstrated by microscopy and flow cytometry.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a4870727cd35935dfc1ac23c87501375 https://doi.org/10.1039/c1jm11948f Zobrazit plný text záznamu