Genetic stability of human endometrial mesenchymal stem cells assessed with morphological and molecular karyotyping
| Autor: | N. N. Nikolsky, Tatyana M. Grinchuk, M. A. Shilina |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetics education.field_of_study Mesenchymal stem cell Isochromosome Population Aneuploidy Karyotype Cell Biology Biology medicine.disease Molecular biology 03 medical and health sciences 030104 developmental biology medicine Stem cell Chromosome breakage education Genomic organization |
| Zdroj: | Cell and Tissue Biology. 11:35-41 |
| ISSN: | 1990-5203 1990-519X |
| Popis: | The aim of this study was to monitor the genetic stability of endometrial mesenchymal stem cells (eMSCs) by G-banding and molecular karyotyping. We evaluated the sensitivity of each method to assess the genetic stability of eMSCs. G-banding karyotyping performed on passages 6 and 15 showed that more than 80% cells had normal karyotype. Random karyotypic changes were found in a small part of the cell population: aneuploidy, isochromosomes, chromosome breakages, interchromosomal association. Molecular karyotyping carried out on the 6th and 14th passages revealed genomic stability, except for in the case of chromosomes 7 and 14. Microduplications 7q36.3 (62 kb) and 14q11.2 (165kb) were found in these chromosomes. We interpreted these aberrations as being derived from the donor of these cells. The morphological and molecular karyotyping complemented each other. Using these methods, we can analyze karyotypic stability at different levels of the genomic organization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink