Radioimmunoassay of Triazolam
| Autor: | J. B. Hester, Karen T. Johnston, Howard Ko, Max E. Royer |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Triazolam
Chromatography food.ingredient Chemistry Cholesterol Biochemistry (medical) Clinical Biochemistry Radioimmunoassay medicine.disease_cause Biochemistry Cross-reactivity Lecithin Analytical Chemistry Standard curve chemistry.chemical_compound Blood serum food Blood plasma Electrochemistry medicine Spectroscopy medicine.drug |
| Zdroj: | Analytical Letters. 10:1019-1040 |
| ISSN: | 1532-236X 0003-2719 |
| Popis: | Two radioimmunoassay (RIA) assay methods (I, II) have been developed for triazolam (T, U-33,030) in serum and plasma. The parameters of equilibration time, serum blank, antibody specificity, extraction efficiency, and drug-binding to glass were studied. Of the various triazolam analogs tested for cross reactivity, only the hydroxy metabolites interfered significantly. At the levels normally found in plasma or serum, a background blank was encountered from constituents such as fatty acids, lysolecithin, lecithin, and cholesterol. However, serum or plasma samples could be analyzed with (I) by constructing standard curves in which blank serum from the same subject was used. An alternate method (II) was found which simultaneously extracted and precipitated the interferences. Both methods could be employed for analysis of plasma or serum samples. However, II detects T metabolites less efficiently than I. The within day and between day coefficients of variation for method I were found to be 5.7% and 3.... |
| Databáze: | OpenAIRE |
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