Application of Thrombin Based Fibrin Glue and Non-Thrombin Based Batroxobin Glue on Intact Human Blood Vessels: Evidence for Transmural Thrombin Activity
| Autor: | William H. Dascombe, Birgit Hessel, Chull Hong, Gregory A. Dumanian, Karyn Labadie, Birger Blombäck, Brian V. Heil, Peter C. Johnson |
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| Rok vydání: | 1997 |
| Předmět: | |
| Zdroj: | Thrombosis and Haemostasis. 78:947-951 |
| ISSN: | 2567-689X 0340-6245 |
| DOI: | 10.1055/s-0038-1657657 |
| Popis: | SummaryAn alternative method of uniting small diameter vessels to obtain tissue union while limiting the thrombogenic effect of suture placement at a vessel anastomosis involves the use of a thrombin based fibrin glue as a surgical sealant. This investigation addresses whether the in vitro application of a thrombin based glue (TG), or batroxobin glue (BG), a non-thrombin based glue made with the snake venom enzyme batroxobin, alters intravascular platelet deposition (PD) or cleaves blood fibrinogen, as measured by fibrinopeptide A (FPA) production, when the respective glue is applied to the external surface of an intact human placental artery or an artery with an anastomosis.When TG was applied to the adventitial surface of an intact vessel or an anastomosis (n = 7) of control and experimental vessels, there was a significant increase in intraluminal platelet deposition, an effect not realized with BG (n = 12, intact vessel TG p = 0.01, BG p = 0.66, anastomosis TG p The data suggest that TG and BG are drug delivery systems for their respective enzymes that either pass through or transfer a message across not only a surgically created anastomosis, but also an intact vessel wall. |
| Databáze: | OpenAIRE |
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