Extracellular Oxidase from the Neonothopanus nambi Fungus as a Promising Enzyme for Analytical Applications
| Autor: | Vladimir S. Bondar, N. O. Ronzhin, Ekaterina Posokhina, O. A. Mogilnaya |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences Oxidase test Chromatography biology 030302 biochemistry & molecular biology Organic Chemistry Flavoprotein Bioengineering Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Enzyme chemistry biology.protein Hydrogen peroxide Biosensor Neonothopanus nambi Mycelium 030304 developmental biology Peroxidase |
| Zdroj: | The Protein Journal. 40:731-740 |
| ISSN: | 1875-8355 1572-3887 |
| Popis: | The extracellular enzyme with oxidase function was extracted from the Neonothopanus nambi luminescent fungus by using mild processing of mycelium with β-glucosidase and then isolated by gel-filtration chromatography. The extracted enzyme is found to be a FAD-containing protein, catalyzing phenol co-oxidation with 4-aminoantipyrine without addition of H2O2, which distinguishes it from peroxidases. This fact allowed us to assume that this enzyme may be a mixed-function oxidase. According to gel-filtration chromatography and SDS-PAGE, the oxidase has molecular weight of 60 kDa. The enzyme exhibits maximum activity at 55–70 °C and pH 5.0. Kinetic parameters Km and Vmax of the oxidase for phenol were 0.21 mM and 0.40 µM min−1. We suggest that the extracted enzyme can be useful to develop a simplified biosensor for colorimetric detection of phenol in aqueous media, which does not require using hydrogen peroxide. |
| Databáze: | OpenAIRE |
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