A Study of Bromine Speciation in Human Serum and Ambroxol Determination in Rat Plasma by Liquid Chromatography–Inductively Coupled Plasma Mass Spectrometry

Autor: Zongli Huo, Hong Liu, Wenling Ji, Deye Liu, Geng Chen, Hualiang Liu
Rok vydání: 2019
Předmět:
Zdroj: Chromatographia. 82:927-934
ISSN: 1612-1112
0009-5893
Popis: In this study, we employed liquid chromatography–inductively coupled plasma mass spectrometry (LC–ICP-MS) to explore bromine bioforms in human serum. Three bromine species were found by size exclusion chromatography–inductively coupled plasma mass spectrometry (SEC–ICP-MS), and one of the most abundant species was identified as Br− by anion exchange chromatography–inductively coupled plasma mass spectrometry (IC–ICP-MS).The quantitative detection of Br− was performed by SEC–ICP-MS, and the total bromine was detected by ICP-MS. Results for the determination of Br− and total bromine in human serum revealed that Br− is the major form of bromine in human serum, with a ratio ranging from 57.3 to 68.8%. Furthermore, we developed a method for the determination of ambroxol based on reversed phase high-performance liquid chromatography–inductively coupled plasma mass spectrometry (RP-HPLC–ICP-MS), and then applied it in rat plasma analysis. After optimization of the RP-HPLC–ICP-MS parameters and pre-treatment procedures, the retention time of ambroxol was 4.6 min and the detection limit was 7.0 μg L−1. The average recovery was 85.4%, with relative standard deviation of 8.4%. The above results showed that the RP-HPLC–ICP-MS method possessed the advantages of low matrix interference and time efficiency, which were better than those reported for traditional RP-HPLC methods. Therefore, this new method for the detection of ambroxol based on RP-HPLC–ICP-MS was certainly a good tool for ambroxol analysis.
Databáze: OpenAIRE
Externí odkaz: