Mechanisms of the influence of adiponectin on apolipoproteins A-1 and B production by human hepatocytes
| Autor: | Galina N. Oleinikova, Vladimir S. Shavva, E. B. Dizhe, Aleksandr D. Denisenko, Dmitriy A. Tanyanskiy |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Gene knockdown Adiponectin Apolipoprotein B biology Chemistry Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Lipogenesis Gene expression biology.protein lipids (amino acids peptides and proteins) Signal transduction Protein kinase A Transcription factor |
| Zdroj: | Medical academic journal. 21:39-45 |
| ISSN: | 2687-1378 1608-4101 |
| DOI: | 10.17816/maj62892 |
| Popis: | The aim of the study was to find out the mechanisms of the adiponectin effect on apolipoproteins (apo) A-1 and B production by human hepatocytes. Materials and methods. The study was performed on the human hepatoma cell line HepG2. The expression of the apoA-1 gene was evaluated at the mRNA level by quantitative PCR with reverse transcription, and the production of apo B by ELISA method. The activity of lipogenesis was assessed by the inclusion of labeled 14C-acetate in triglycerides, as well as by mRNA expression of lipogenesis genes, and by the estimation of total triglycerides content in cells. To determine the involvement of signaling pathways, the RNA interference method was used. Results. Knockdown of genes, coding the specific receptors, AMP-activated protein kinase, and its regulated transcription factors inhibited adiponectin-dependent stimulation of apoA-1 gene expression in hepatocytes. Adiponectin had no effect on lipogenesis and apo B production under basal conditions, but suppressed these processes induced by the addition of oleate. Conclusion. Adiponectin stimulates the production of apo A-1 in hepatocytes by inducing the transcription of the apoA-1 gene and suppresses the secretion of apo B by affecting lipogenesis. These effects may underlie the effect of adiponectin on lipoproteins metabolism. |
| Databáze: | OpenAIRE |
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