| Popis: |
The level of glutathione in human are closely related to various diseases. In this paper, a sensitive “Turn-On” sensor for glutathione is reported. The sensor was designed based on fluorescence resonance energy transfer (FRET) between fluorescein isothiocyanate (FITC) and gold nanoparticles (AuNPs). Energy transfer form donor (FITC) to acceptors (AuNPs) because of the electrostatic absorption, resulting in the quenching of FITC fluorescence. With the addition of glutathione, FITC detach from the surface of AuNPs, because of the strong force of Au-S bond. The distance between the FITC and AuNPs increased, the fluorescence of FITC will restore because of the destruction of FRET process. Based on this principle, we construct the “Turn-On” sensor to detect GSH and give fluorescence tests in different buffers and pH values.The results revealed that the neutral PBS buffer environment is the most suitable environment for sensor. The fluorescence intensity of system decrease gradually with the decreasing concentration of GSH. The linear range of glutathione concentrations in the detection system is 1~650mg/L. We obtain a satisfying result and it can be used to detect the high concentrations of glutathione content in human body environment. |
