Grape Powder Supplementation Prevents Oxidative Stress–Induced Anxiety-Like Behavior, Memory Impairment, and High Blood Pressure in Rats
| Autor: | Mohammad Asghar, Farida Allam, An T. Dao, Samina Salim, Christopher Mowrey, Karim A. Alkadhi, Ritu Bohat, Gaurav Chugh, Faizan Jafri, Gaurav Patki |
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| Rok vydání: | 2013 |
| Předmět: |
Brain-derived neurotrophic factor
medicine.medical_specialty Nutrition and Dietetics biology Chemistry Glutathione reductase Medicine (miscellaneous) chemistry.chemical_element Glutathione Calcium CREB medicine.disease_cause Lactoylglutathione lyase chemistry.chemical_compound Endocrinology Internal medicine biology.protein medicine Buthionine sulfoximine Oxidative stress |
| Zdroj: | The Journal of Nutrition. 143:835-842 |
| ISSN: | 0022-3166 |
| DOI: | 10.3945/jn.113.174649 |
| Popis: | We examined whether or not grape powder treatment ameliorates oxidative stress-induced anxiety-like behavior, memory impairment, and hypertension in rats. Oxidative stress in Sprague-Dawley rats was produced by using L-buthionine-(S,R)-sulfoximine (BSO). Four groups of rats were used: 1) control (C; injected with vehicle and provided with tap water), 2) grape powder-treated (GP; injected with vehicle and provided for 3 wk with 15 g/L grape powder dissolved in tap water), 3) BSO-treated [injected with BSO (300 mg/kg body weight), i.p. for 7 d and provided with tap water], and 4) BSO plus grape powder-treated (GP+BSO; injected with BSO and provided with grape powder-treated tap water). Anxiety-like behavior was significantly greater in BSO rats compared with C or GP rats (P < 0.05). Grape powder attenuated BSO-induced anxiety-like behavior in GP+BSO rats. BSO rats made significantly more errors in both short- and long-term memory tests compared with C or GP rats (P < 0.05), which was prevented in GP+BSO rats. Systolic and diastolic blood pressure was significantly greater in BSO rats compared with C or GP rats (P < 0.05), whereas grape powder prevented high blood pressure in GP+BSO rats. Furthermore, brain extracellular signal-regulated kinase-1/2 (ERK-1/2) was activated (P < 0.05), whereas levels of glyoxalase-1 (GLO-1), glutathione reductase-1 (GSR-1), calcium/calmodulin-dependent protein kinase type IV (CAMK-IV), cAMP response element-binding protein (CREB), and brain-derived neurotrophic factor (BDNF) were significantly less (P < 0.05) in BSO but not in GP+BSO rats compared with C or GP rats. We suggest that by regulating brain ERK-1/2, GLO-1, GSR-1, CAMK-IV, CREB, and BDNF levels, grape powder prevents oxidative stress-induced anxiety, memory impairment, and hypertension in rats. |
| Databáze: | OpenAIRE |
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