Poly(adenosine diphosphate-ribose) synthesis in transformed mouse epidermal cells in culture
| Autor: | Toshihiro Tanaka, Kunihiro Ueda, Kouichi Ikai |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
chemistry.chemical_classification
Histology Epidermis (botany) Physiology NAD+ ADP-Ribosyltransferase Retinoic acid Cell Biology Biology Biochemistry Adenosine Molecular biology Pathology and Forensic Medicine chemistry.chemical_compound Enzyme chemistry Biosynthesis Cell culture medicine Incubation medicine.drug |
| Zdroj: | ACTA HISTOCHEMICA ET CYTOCHEMICA. 24:251-255 |
| ISSN: | 1347-5800 0044-5991 |
| DOI: | 10.1267/ahc.24.251 |
| Popis: | The activity of poly(adenosine diphosphate-ribose) (poly(ADP-ribose)) synthesis was examined in permeabilized transformed mouse epidermal cells (PAM 212 cells) in culture. PAM cells grown in low (0.02mM) Ca++ medium showed a higher activity of poly(ADP-ribose) synthesis compared with the cells grown in normal (0.7mM) Ca++ medium. Poly(ADP-ribose) synthesis was completely inhibited by 1mM 3-aminobenzamide, which is a specific inhibitor of poly(ADP-ribose) synthetase. PAM 212 cells grown in medium containing 2% fetal calf serum (FCS) switched from medium containing 15% FCS showed an increase of poly(ADP-ribose) synthesizing activity time-dependently during incubation. After PAM cells grown in normal Ca++ medium were cultivated with 1α, 25-dihydroxyvitamin D3 (1α, 25-(OH)2-D3) or TPA, poly(ADP-ribose) synthesizing activity decreased time-dependently, compared with the condition without addition, but an incubation with all-trans-retinoic acid (retinoic acid) did not show such an effect. Immunohistochemical analysis using anti-poly(ADP-ribose) antibody confirmed the increased poly(ADP-ribose) synthesis in nuclei of PAM cells grown in low Ca++ medium. These results suggest that poly(ADP-ribose) synthesizing activity is correlated to proliferation and differentiation of epidermal cells. |
| Databáze: | OpenAIRE |
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