| Autor: | R. Geertz, Guenther Gercken, Wieland Kiess, Attila Tarnok, Andreas Hoeflich, Ulrike Kessler |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
chemistry.chemical_classification
Antiserum Growth factor medicine.medical_treatment Clinical Biochemistry food and beverages Cell Biology General Medicine Insulin-Like Growth Factor Receptor Biology Fluorescence Blot chemistry.chemical_compound chemistry Biochemistry medicine Inositol Receptor Inositol phosphate Molecular Biology |
| Zdroj: | Molecular and Cellular Biochemistry. 177:33-45 |
| ISSN: | 0300-8177 |
| Popis: | Expression of insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6-phosphate (IGF-II/Man6P) receptors in cultured bovine alveolar macrophages (BAM) was demonstrated by competitive binding studies and crosslinking experiments. Western blotting of protein extracts from cultured BAM using an anti bovine IGF-II/Man6P receptor antiserum (#66416) confirmed the presence of IGF-II/Man6P receptors on BAM. The effects of IGFs and Man6P on generation of inositol phosphates was measured by HPLC analysis of perchloric acid extracts from myo-[3H]inositol-labelled cultured BAM. IGF-I at nanomolar concentrations and Man6P (10-8−103 M) stimulated the accumulation of both Ins(1,4,5)P3 and Ins(1,3,4,5)P4 after 30 sec. IGF-II (up to 2.3 × 10-8M) had no significant effect on inositol phosphate accumulation under the same conditions. Both IGFs and Man6P induced a rise in [Ca2+]iin cultured BAM. In addition, using the fluorescent dye SNARF-1/AM we could demonstrate rapid but small IGF-II (10-9 M) triggered acidification (0.07 pH units) of cultured BAM. Taken together, our results indicate not only the presence of both IGF-I and IGF-II/Man6P receptors on BAM, but also provide evidence of the linkage of the IGF-I receptor to the inositol phosphate system. |
| Databáze: | OpenAIRE |
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