Effective degradation of aflatoxin B1 using a novel thermophilic microbial consortium TADC7
| Autor: | Yi Wang, Zongjun Cui, Chunxia Zhao, Peng Guo, Wei Cheng, Yucai Lyu, Dongdong Zhang, Mingming Zhao, Dan Zheng |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Aflatoxin Environmental Engineering Renewable Energy Sustainability and the Environment Toxin Thermophile 030106 microbiology Bioengineering General Medicine Biodegradation Microbial consortium medicine.disease_cause Geobacillus 03 medical and health sciences 030104 developmental biology Enzyme chemistry Chemical engineering medicine Degradation (geology) Food science Waste Management and Disposal |
| Zdroj: | Bioresource Technology. 224:166-173 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2016.11.033 |
| Popis: | We constructed a novel thermophilic microbial consortium, TADC7, with stable and efficient aflatoxin B1 (AFB1) degradation activity. The microbial consortium degraded more than 95% of the toxin within 72h when cultured with AFB1, and the optimum temperature was 55-60°C. TADC7 tolerated high doses of AFB1, with no inhibitory effects up to 5000μgL-1 AFB1; moreover, the degradation kinetics fit well with the Monod model. The proteins or enzymes in the TADC7 cell-free supernatant played a major role in AFB1 degradation. AFB1 degradation by the cell-free supernatant was stable up to 90°C, with an optimal pH of 8-10. We performed 16S rRNA sequencing to determine TADC7 community structure dynamics; the results indicated that Geobacillus and Tepidimicrobium played major roles in AFB1 degradation. |
| Databáze: | OpenAIRE |
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