A high-throughput electrochemical impedance spectroscopy evaluation of bioresponsibility of the titanium microelectrode array integrated with hydroxyapatite and silver
| Autor: | Fan Zhang, Longxiang Lin, Guo-Wei Wang, Changjian Lin, Yong Chen, Ren Hu |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Materials science
General Chemical Engineering Analytical chemistry Oxide chemistry.chemical_element 02 engineering and technology Multielectrode array 010402 general chemistry 021001 nanoscience & nanotechnology Electrochemistry 01 natural sciences 0104 chemical sciences Dielectric spectroscopy chemistry.chemical_compound Chemical engineering chemistry Equivalent circuit 0210 nano-technology Layer (electronics) Titanium Protein adsorption |
| Zdroj: | Electrochimica Acta. 85:152-161 |
| ISSN: | 0013-4686 |
| DOI: | 10.1016/j.electacta.2012.08.033 |
| Popis: | a b s t r a c t This paper reports a transparent Ti microelectrode array (MEA) system for a high-throughput evaluation of bioresponsibility using electrochemical impedance spectroscopy (EIS). The MEA chip integrated with hydroxyapatite (HA) and Ag coatings was selectively prepared by electrochemical deposition based on a novel procedure of multichannel current control. The EIS measurement of living MG63 osteosarcoma cells in the integrated MEA chip was conducted, and the result was analyzed using an equivalent circuit corresponding to a titanium oxide film, protein adsorption layer, cell adhesion layer, and medium. It is shown that the bioresponsibility of Ti-Ag-HA on the MEA chip can be improved, compared with the Ti, Ti-HA, and Ti-Ag coatings. The system was further used for real-time EIS monitoring during continuous cell culture for a long period (12 days). The effect of the long-term cell proliferation on the EIS behavior was discussed. This integrated system is valuable to significantly simplify the operation procedures and quickly evaluate the bioresponsibility of biomaterials. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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