Development and characterization of nine microsatellite loci from the Korean hare (Lepus coreanus) and genetic diversity in South Korea

Autor:	Yun-Sun Lee, Junghwa An, Junpei Kimura, Hang Lee, Mi-Sook Min, Sungkyoung Choi, Sang-In Kim, Han-Chan Park, Kyung-Seok Kim
Rok vydání:	2012
Předmět:	Genetics Core set Genetic diversity Microsatellite Population genetics Zoology Animal Science and Zoology Locus (genetics) Korean hare Biology Allele biology.organism_classification General Biochemistry Genetics and Molecular Biology
Zdroj:	Animal Cells and Systems. 16:230-236
ISSN:	2151-2485 1976-8354
DOI:	10.1080/19768354.2011.640351
Popis:	The Korean hare, Lepus coreanus, is an important mammal in ecosystem food chains, and is distributed across the entire Korean peninsula and northeastern China. Polymorphic microsatellite loci were developed using the biotin-enrichment technique for use in population genetics studies. Five trinucleotide and four dinucleotide microsatellite loci were selected and tested on 22 Korean hare specimens collected from Gangwon Province and Gyeongsangbuk Province in South Korea. The number of alleles across the two sampling regions ranged from three to nine with a mean of 6.1. Mean observed and expected heterozygosities and polymorphic information content were 0.540, 0.627 and 0.579, respectively. Only one locus, Lc06, showed departure from Hardy-Weinberg equilibrium after applying the Bonferroni correction. Four microsatellites, Lc01, Lc03, Lc12, and Lc19, satisfied the criteria to serve as a core set of markers recommended for population genetics studies. These new microsatellite markers will be widely a...
Databáze:	OpenAIRE
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