Change of multidrug resistance and sensitivity of leukemic cells to oxidative stress in large tumors

Autor: A. F. Korystova, Yu. A. Kim, Maksim O. Emel’yanov, Vera V. Shaposhnikova, M. Kh. Levitman, Yu. N. Korystov
Rok vydání: 2010
Předmět:
Zdroj: Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology. 4:286-292
ISSN: 1990-7494
1990-7478
DOI: 10.1134/s1990747810030062
Popis: The possibility of modification of vincristine-resistant strain of leukemic cells upon long-term tumor transplantation was studied. DBA2 mice were inoculated with cells P388VR, a vincristine-resistant strain of leukemic cells P388 either from small 5-day-old tumors or from large 7 to 8-day-old tumors. Multidrug resistance (MDR) of the cells was evaluated by cell sensitivity to vincristine and from the transport of calcein ester out of cells. Sensitivity of cells to oxidative stress was determined by cell viability and the production of reactive oxygen species in cells, by dichlorofluorescein assay. It has been shown that properties of the P388VR strain did not change in mice inoculated with the cells from small tumors. However, if the cells from large tumors were used for inoculation, the strain underwent the following modifications: tumors became contaminated with erythrocytes; resistance to vincristine increased; transport of calcein ester into and out of the cells accelerated, and sensitivity to oxidative stress raised. These changes in the properties of the P388VR strain persisted upon consequent propagation of the tumor and could be caused by a constitutive activation of the HIF system. The possibility of the alterations of the leukemic cells upon tumor transplantation from large tumors has to be taken into account in the experimental work with tumor cell lines.
Databáze: OpenAIRE
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