Development of polymorphic microsatellite from RAPD bands of black sea bream Acanthopagrus schlegeli

Autor:	Yundan Liu, Z. X. Li, Duobing Wang, Hong Lin, A. Y. Gao, L. X. Liu, Bafang Li
Rok vydání:	2007
Předmět:	Genetics Black sea bream Ecology Fish species Acanthopagrus schlegeli Microsatellite Polymorphic locus Primer (molecular biology) Biology Sequence repeat Biochemistry General Biochemistry Genetics and Molecular Biology RAPD
Zdroj:	Molecular Ecology Notes. 7:1178-1180
ISSN:	1471-8286 1471-8278
DOI:	10.1111/j.1471-8286.2007.01823.x
Popis:	Eight pairs of simple sequence repeat markers were developed from random amplified polymorphic DNA product in black sea bream Acanthopagrus schlegeli. Twenty microsatellites were selected for designing microsatellite primers, of which eight gave working primer pairs. They had between three and seven alleles. Observed and expected heterozygosities varied from 0.65 to 0.90, and from 0.58 to 0.82, respectively. Eight additional fish species assessed for cross-species amplification revealed between two and five positive amplifications and between zero and three polymorphic loci per species.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a422cfb5e1e63cda52ccaca1552bd2d7 https://doi.org/10.1111/j.1471-8286.2007.01823.x Zobrazit plný text záznamu Plný text