Immunoradiometric assay for baculovirus enveloped nucleocapsids and polyhedrin

Autor:	Pepper Hoops, Michio Ohba, Gale E. Smith, Max D. Summers
Rok vydání:	1977
Předmět:	Antiserum Immunoradiometric assay biology viruses fungi biology.organism_classification Virology Molecular biology Virus Autographa californica Antigen Cell culture biology.protein Polyhedrin Antibody Ecology Evolution Behavior and Systematics
Zdroj:	Journal of Invertebrate Pathology. 30:362-368
ISSN:	0022-2011
DOI:	10.1016/0022-2011(77)90146-x
Popis:	A solid-phase microtiter immunoradiometric assay was developed for Autographa californica enveloped nucleocapsids purified from polyhedra, nonoccluded virus from cell culture, and purified polyhedrin. Comparative studies with antigens and antisera from each source demonstrate that micro-solid-phase immunoradiometric assay was specific within the range of 10–200 ng for the enveloped nucleocapsids and from 200 pg to 200 ng for polyhedrin. A preliminary study also demonstrated the ability to use this assay to detect specific antiviral antibodies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a42230e945ee27569a212b7604fcaf0f https://doi.org/10.1016/0022-2011(77)90146-x Zobrazit plný text záznamu