Molecular detection of Leishmania (Sauroleishmania) tarentolae in human blood and Leishmania (Leishmania) infantum in Sergentomyia minuta : unexpected host‐parasite contacts

Autor:	A. Giacomi, Simona Gabrielli, Giulia Barlozzari, Gladia Macrì, Domenico Otranto, Jairo Alfonso Mendoza-Roldan, Marco Pombi
Rok vydání:	2020
Předmět:	0301 basic medicine General Veterinary biology 030231 tropical medicine Kinetoplastida Leishmaniasis 030108 mycology & parasitology biology.organism_classification Blood meal medicine.disease Leishmania Microbiology law.invention 03 medical and health sciences 0302 clinical medicine law Insect Science Vector (epidemiology) parasitic diseases medicine Parasite hosting Parasitology Leishmania infantum Ecology Evolution Behavior and Systematics Polymerase chain reaction
Zdroj:	Medical and Veterinary Entomology. 34:470-475
ISSN:	1365-2915 0269-283X
Popis:	The detection of atypical Kinetoplastida in vertebrate hosts and vectors might suggest unexpected host-parasite contacts. Aside to major vectors of Leishmania (Leishmania) infantum in Italy (e.g. Phlebotomus perniciosus and Phlebotomus perfiliewi), the sand fly fauna also includes Sergentomyia minuta, herpetophilic and proven vector of Leishmania (Sauroleishmania) tarentolae, in which records of blood meal on mammals and detection of L. infantum DNA are increasing. This study was conducted in Central Italy aiming to molecularly detect potential atypical Leishmania host-vector contacts. Detection of Leishmania spp. DNA was performed by polymerase chain reaction (SSU rRNA, ITS1 targets) on field-collected sand fly females (N = 344), blood samples from humans (N = 185) and dogs (N = 125). Blood meal identification was also performed on engorged sand flies. Leishmania spp. DNA was found in 13.1% sand flies, 3.7% humans and 14.4% dogs. Sequence analysis identified L. infantum in S. minuta (4.4%), P. perniciosus (9.1%), humans (2.2%) and dogs (14.4%). Leishmania tarentolae was detected in S. minuta (12.6%), P. perfiliewi (6.6%) and human (1.6%) samples. Of 28 S. minuta examined for blood meal, 3.6 and 21.4% scored positive for human and lizard DNA, respectively. These results indicate the importance of one-health approach to explore new potential routes of transmission of leishmaniasis involving S. minuta.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a41d282901ed21af45374589cf10f73e https://doi.org/10.1111/mve.12464 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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