Popis: |
Giardia duodenalis is a common intestinal parasite in humans, a wide range of domesticated and wild animals. There are human and animal specific, as well as zoonotic pathogenic genotypes. It is not clear whether livestock in close proximity to humans could be infected with human specific genotypes, and vice versa. In this study, Giardia-positive faecal samples were collected from both humans (n = 4) (from Maamobi Polyclinic in the Ayawaso Sub- Metro) and calves (n = 8) (from Animal Research Institute Farms, Adentan Municipality), in Ghana. Nested PCR amplification using Giardia-specific, Glutamate dehydrogenase (GDH) genes and Triosephosphate isomerase (TPI) gene primers for human and animal faecal samples respectively was carried out. Results showed that 100% of the calves carried the TPI-B genotype, which is a common pathogenic genotype in humans. This report is based on the unusual results obtained as Giardia duodenalis genotype B is known to occur in humans but is being observed in calves for the first time. This suggests that calves in close proximity to humans could be reservoirs and sources of human Giardia infections. |