Detection of point mutations in T lymphocytes

Autor:	Georges R. Mohn, A.A. van Zeeland, Paul H.M. Lohman, Jacob G. Jansen, Ad D. Tates, Harry Vrieling
Rok vydání:	1995
Předmět:	DNA damage DNA repair Point mutation Biochemistry (medical) Clinical Biochemistry Biology Molecular biology Reverse transcription polymerase chain reaction chemistry.chemical_compound Adductomics chemistry Complementary DNA Gene duplication DNA
Zdroj:	Clinical Chemistry. 41:1841-1843
ISSN:	1530-8561 0009-9147
Popis:	We exposed experimental animals to a series of alkylating agents that induced mutations at the X-linked hprt gene of T lymphocytes. We then isolated the mutant cells and analyzed the molecular nature of the mutations by amplification of hprt cDNA sequences with the use of reverse transcriptase PCR followed by DNA sequence analysis, and then correlated the mutational spectra obtained to the spectra of DNA adducts caused by the alkylating agents used. The nature of the base-pair changes causing the mutations was characteristic for the reaction pattern of the genotoxic agent with DNA. However, we also found a clear influence of DNA repair processes; i.e., in those cells that were able to remove certain types of DNA damage, the class of mutations expected from that type of damage was reduced.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a3ea5c3ada55036afb1adcd50d235e52 https://doi.org/10.1093/clinchem/41.12.1841 Zobrazit plný text záznamu