Bcl-2 expression correlates positively with serum basic fibroblast growth factor (bFGF) and negatively with cellular vascular endothelial growth factor (VEGF) in patients with chronic lymphocytic leukaemia
| Autor: | Osnat Bairey, Matityahu Shaklai, Ester Rabizadeh, Yael Zimra |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
medicine.diagnostic_test Angiogenesis Basic fibroblast growth factor Hematology Biology Vascular endothelial growth factor chemistry.chemical_compound Endocrinology medicine.anatomical_structure chemistry Western blot Downregulation and upregulation Apoptosis Cell culture Internal medicine White blood cell medicine |
| Zdroj: | British Journal of Haematology. 113:400-406 |
| ISSN: | 0007-1048 |
| Popis: | A large proportion of B-chronic lymphocytic leukaemia (B-CLL) cells express the anti-apoptotic protein Bcl-2. Basic fibroblast growth factor (bFGF) has been shown to upregulate the expression of Bcl-2 in B-CLL cell lines. Vascular endothelial growth factor (VEGF) has been shown to enhance the survival of endothelial cells by upregulating the expression of Bcl-2. In the present study, we measured serum and cellular levels of bFGF and VEGF in 85 patients with CLL using a commercial quantitative sandwich enzyme immunoassay technique. Levels of Bcl-2 were also assayed concomitantly using Western blot analysis. The mean serum level of bFGF was 53·4 pg/ml (range 0–589) and that of VEGF 459·2 pg/ml (range 33–1793). The mean cellular level of bFGF was 158·3 pg/2 × 105 cells (range 0·8–841) and VEGF, 42·4 pg/2 × 105 cells (range 0–244). A high correlation was found between serum and cellular bFGF levels (P |
| Databáze: | OpenAIRE |
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