Preventive effect of intravesical ozone supplementation on n-methyl-n-nitrosourea-induced non-muscle invasive bladder cancer in male rats
| Autor: | Demir Kursat Yildiz, Meltem Ozlen Dillioglugil, Hasan Yilmaz, Muhammed E Keles, Oguz Ozden Cebeci, Tayyar Alp Ozkan, Levend Ozkan, Kerem Teke, Ozdal Dillioglugil |
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| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
Bladder cancer General Veterinary biology business.industry medicine.medical_treatment 030232 urology & nephrology Urology General Medicine medicine.disease General Biochemistry Genetics and Molecular Biology Superoxide dismutase 03 medical and health sciences 0302 clinical medicine Every other week 030220 oncology & carcinogenesis Male rats biology.protein N-Methyl-N-nitrosourea Medicine Animal Science and Zoology Urothelium Non muscle invasive business Saline |
| Zdroj: | Experimental Animals. 66:191-198 |
| ISSN: | 1881-7122 1341-1357 |
| Popis: | Although non-muscle invasive bladder cancer (NMIBC) is widely seen in men, most laboratory studies of new intravesical therapies to prevent NMIBC have been conducted on female animals. In addition, ozone (O3) has been shown to be a beneficial agent as an intravesical application in the treatment of various disorders. In the current study, we evaluated the immunohistopathological and oxidative-antioxidative effects of intravesical O3 treatment on n-methyl-n-nitrosourea (MNU)-induced NMIBC. Male Wistar-Albino rats (n=51) were divided into four groups: sham (n=6), O3 only (n=15), MNU only (n=15), and MNU+O3 (n=15). The MNU-only and MNU+O3 groups received MNU, and the O3-only group received saline every other week for 10 weeks. The MNU-only group received 1 ml saline in place of O3 treatment, whereas the O3-only and MNU+O3 groups were treated with 1 ml 25 µg/ml O3 between the 7th and 12th weeks. Rat bladders were collected in the 15th week for immunohistopathology and oxidant-antioxidant quantitation. Oxidant-antioxidant parameters were determined by ELISA. Although all surviving rats in the MNU-only group had preneoplastic (4/11, 36.4%) or neoplastic changes (7/11, 63.6%), a completely normal urothelium was observed in 2 rats (2/12, 16.7%) in the MNU+O3-group (P=0.478). More high-grade lesions were observed in the MNU-only group (4/11, 36.4%) than in the MNU+O3 group (1/12, 8.3%) (P=0.120). All oxidant-antioxidant parameters significantly increased (P |
| Databáze: | OpenAIRE |
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