Comparison of barley seed proteomic profiles associated with fusarium head blight reaction

Autor:	K. Xi, J. Zantinge, Michael Johns, J. H. Helm, Tineke H. Jones, A. Murray, K. Kumar, Patricia Juskiw
Rok vydání:	2010
Předmět:	Fusarium Gel electrophoresis biology food and beverages Plant Science Plant disease resistance biology.organism_classification Botany Proteome Blight Poaceae Hordeum vulgare Hordeum Agronomy and Crop Science
Zdroj:	Canadian Journal of Plant Pathology. 32:496-512
ISSN:	1715-2992 0706-0661
DOI:	10.1080/07060661.2010.529252
Popis:	Fusarium head blight (FHB) incited primarily by Fusarium graminearum Schwabe, is a destructive disease that affects the quality and yield of barley (Hordeum vulgare L.) grain. At present, there is a limited understanding of the molecular mechanisms involved in barley resistance against infection by and the spread of F. graminearum. The objective of the current study was to identify proteins that were differentially expressed in FHB susceptible and resistant barley accessions. Mature seed from six barley accessions and five pairs of barley sister lines differing in FHB reactions were subject to proteome analysis by two-dimensional gel electrophoresis (2DE). Coomassie Brilliant Blue staining visualized about 1000 seed protein spots of pI 4–7 on 2DE gels. Proteins were identified by liquid chromatography and tandem mass spectrometry (LC-MS/MS) sequencing after in-gel digestion by trypsin. In our first study, we compared the 2DE seed protein profiles of two FHB susceptible varieties ‘AC Lacombe’ and ...
Databáze:	OpenAIRE
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