Multianalysis of Thyroid Tumor Markers on the Surface of a Porous Membrane and Semiconductor Substrates using Gold Nanoparticles as a Label
| Autor: | Vladimir A. Krupenin, Alexey M. Egorov, Denis E. Presnov, M. Yu. Rubtsova, G. V. Presnova, M. M. Ulyashova |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Detection limit endocrine system Analyte Chromatography Chemistry medicine.drug_class medicine.medical_treatment 010401 analytical chemistry Substrate (chemistry) Nanoparticle General Chemistry Monoclonal antibody 01 natural sciences 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Colloidal gold medicine Thyroglobulin Nitrocellulose |
| Zdroj: | Moscow University Chemistry Bulletin. 73:173-178 |
| ISSN: | 1935-0260 0027-1314 |
| DOI: | 10.3103/s0027131418040089 |
| Popis: | We present a method for the simultaneous detection of thyroglobulin and thyroid-stimulating hormone on microchips using gold nanoparticles for antibody labeling. Nitrocellulose and silicon were used as supports for the microchips. The detection principle was based on a sandwich immunoassay using a pair of antibodies that simultaneously bind to the analyte. Specific monoclonal antibodies that are most sensitive and specific in detecting the hormones and signal-to-background ratio are selected. The analytical signal was the intensity of the staining of the microchip zones on the membrane and the nanoparticle density on the silicon substrate. The detection limit for thyroglobulin and thyroid-stimulating hormone was 0.25 ng/mL and 0.016 μIU/mL for the membrane support and 0.1 ng/mL and 0.013 μIU/mL for silicon substrate, respectively. The method can be further used to develop nanoscale sensor devices for the express diagnostics of thyroid pathologies. |
| Databáze: | OpenAIRE |
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