Self-assembly of Filamentous Cell Penetrating Peptides for Gene Delivery
| Autor: | Dawei Xu, He Dong, Louis B. DeRidder, Brendan Elmore |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Small interfering RNA
Chemistry Cell macromolecular substances 02 engineering and technology Plasma protein binding Transfection Gene delivery 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Supramolecular assembly medicine.anatomical_structure Biophysics Membrane activity Cell-penetrating peptide medicine 0210 nano-technology |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493978090 |
| DOI: | 10.1007/978-1-4939-7811-3_17 |
| Popis: | Cell penetrating peptides (CPPs) have been proven to be an effective vector to deliver a variety of membrane-impermeable macromolecules, such as DNAs, siRNAs, and proteins. Conventional single-chain CPPs typically suffer from severe protease degradation and fast clearance rate for in vivo therapeutic delivery application. In this chapter, we show that supramolecular assembly of de novo designed cationic multidomain peptides (MDPs) leads to nanostructured filaments with increased proteolytic stability and potent membrane activity necessary for improved transfection efficiency. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|