Treatment with Insulin-Like Growth Factor-1 Increases Chondrogenesis by Periosteum In Vitro
| Autor: | David R. Diduch, Cay M. Mierisch, Peggy Anderson, Gary Balian |
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| Rok vydání: | 2002 |
| Předmět: |
Periosteum
Chemistry medicine.medical_treatment Type II collagen Cell Biology Anatomy musculoskeletal system Chondrogenesis Biochemistry Chondrocyte Cell biology Insulin-like growth factor medicine.anatomical_structure Rheumatology Gene expression medicine Orthopedics and Sports Medicine Molecular Biology Hyaline Aggrecan |
| Zdroj: | Connective Tissue Research. 43:559-568 |
| ISSN: | 1607-8438 0300-8207 |
| DOI: | 10.1080/03008200290001285 |
| Popis: | The repair of defects in articular cartilage with hyaline tissue that is resilient to wear is a challenging problem. Fibrocartilaginous tissue forms in response to injury through the articular surface and degenerates under mechanical load. Because periosteum contains cells, which are capable of synthesizing cartilage matrix proteins, it has been used to repair defects in articular surfaces. Treatment of periosteal grafts with growth factors, particularly those that elicit chondrocyte gene expression, may improve tissue regeneration. Gene expression by periosteal explants in vitro was measured. Expression of type II collagen and aggrecan mRNA was increased in response to treatment with IGF-I. Furthermore, IGF-I treatment caused an increase in type II collagen and aggrecan mRNA that was time and concentration dependent. The effect of short and long-term (continuous) incubations was compared to determine if a pretreatment could be used to condition a graft for subsequent surgical use. Short-term incubation i... |
| Databáze: | OpenAIRE |
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