Recovery from bone loss, diminished mineral density and strength in mice after treatment with steroidal and nonsteroidal anti-inflammatory drugs by injection of exosomes enriched with agomir miRNAs
| Autor: | Paweł P. Jagodziński, Tomasz P. Lehmann, Maciej Głowacki, Ewa Pruszyńska-Oszmałek, Celina Pezowicz, A. Trzaskowska, Magdalena Wojtków, Paweł Kołodziejski, Mirosław Szybowicz, Sławomir Mielcarek, Marek Nowicki |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Bone mineral Drug medicine.drug_class business.industry media_common.quotation_subject Bone healing Pharmacology Anti-inflammatory Microvesicles 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Diclofenac Methylprednisolone Gene expression medicine 030212 general & internal medicine business media_common medicine.drug |
| Zdroj: | Journal of Medical Science. 88:261-266 |
| ISSN: | 2353-9801 2353-9798 |
| Popis: | The project “Recovery from bone loss, diminished mineral density and strength in mice after treatment with steroidal and nonsteroidal anti-inflammatory drugs by injection of exosomes enriched with agomir miRNAs” is an animal experiment project, and an attempt to apply small RNA strands to reverse the harmful effects of anti-inflammatory drugs. Patients chronically treated with an anti-inflammatory drug suffer from musculoskeletal side effects, including reversed mineralisation and disabled bone fracture healing. The aims of the study are to measure changes in bone mineral density and bone strength in mice treated with methylprednisolone or diclofenac in combination with treadmill exercise. The reversal of the negative effects of these drugs will be assayed using modified miRNA agomir. Bones obtained from the treated mice will be analysed using micro-CT, dynamic mechanical analysis (DMA), nanoindentation, Raman spectroscopy and gene expression. We expect to find specific miRNA counteracting the demineralisation of the mice bones caused by methylprednisolone or diclofenac. |
| Databáze: | OpenAIRE |
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