HLA-D Typing by Homozygous Typing Cells
| Autor: | Eva Bjørn Jensen, Tom Kristensen, F. Jørgensen, L. U. Lamm |
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| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Tissue Antigens. 10:83-98 |
| ISSN: | 1399-0039 0001-2815 |
| Popis: | Several normalization procedures have been applied to single MLC-typing experiments in order to neutralize experimental variation and hence to secure assignment of HLA-D phenotypes. The present paper concerns a statistical description and isolation of experimental and biological factors in repeated MLC-typing experiments. The statistical model used gives a meaningful description of the variables in MLC as judged by its application to MLC-typing data of HLA-identical siblings. Subsequent testing of the model in an HLA-A, -B, -C and -D-genotyped family material provides evidence that the level of typing response to selected homozygous typing cells may be influenced by factors coded for by the homologous HLA-D region. It is, however, concluded that such evidence must be accepted with caution due to the (for the present statistical purpose) suboptimal design for the HLA-D typing experiments and hence the relative insensitivity of the method. A general method for locating erroneous triplicates is devised. |
| Databáze: | OpenAIRE |
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