Production of Ready-to-Reconstitute Functional Beverages by Utilizing Whey Protein Hydrolysates and Probiotics
| Autor: | Yoh Chang Yoon, Soo-Ki Kim, Hyun-Dong Paik, Heddur Manjappa Jayaprakasha, Song Ee Han, Sabbini Kalyan Kumar, A-Ram Jeong |
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| Rok vydání: | 2010 |
| Předmět: |
Whey protein
Chromatography Hydrolyzed protein Bifidobacterium bifidum Chemistry ved/biology ved/biology.organism_classification_rank.species Ultrafiltration food and beverages Hydrolysate chemistry.chemical_compound Lactobacillus acidophilus Animal Science and Zoology Food science Sugar Citric acid Food Science |
| Zdroj: | Korean Journal for Food Science of Animal Resources. 30:575-581 |
| ISSN: | 1225-8563 |
| Popis: | This investigation was aimed at developing a ready-to-reconstitute beverage by utilizing probiotics and whey protein hydrolysates carrying bioactive peptides. Cheddar cheese whey was ultrafiltered. The 18% protein retentate was subjected to protein hydrolysis using Neutrase. The hydrolyzed retentate was further condensed to 35% total solids and spray-dried at 75oC outlet air temperature. Different levels of sugar, citric acid and stabilizer were blended for spray-dried hydrolysates. Spray-dried hydrolysate was further inoculated with different levels of probiotics grown in a whey medium and dried in fluidized-bed drier at 40oC to obtain a ready-to-reconstitute beverage. Hydrolysis was greatest at an enzyme:substrate ratio of 1:25 for 3 h. Spray-dried hydrolysate reconstituted to 1% protein and blended with 15% sugar, 0.2% citric acid and 0.15% xantham gum resulted in a superior product with no sedimentation. Accordingly, sugar, citric acid and xanthum gum were dry-blended with spray-dried hydrolysates. Bifidobacterium bifidum and Lactobacillus acidophilus that was grown separately in a whey medium, blended to produce 2% spray-dried hydrolysate and dried as described above resulted in a readyto-reconstitute beverage mix. The fluidized dried product typically exhibited a probiotic count of 108colony forming units (CFU)/g. However, blending of probiotic to the retentate and direct spray-drying precipitously reduced the probiotic count to 104 CFU/g of powder. |
| Databáze: | OpenAIRE |
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