Phorbol esters and calcium ionophores differentially regulate the transcription of gamma-T-cell antigen receptor gene in human thymocytes
| Autor: | H Martinez-Valdez, E Thompson, A Cohen |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
medicine.medical_specialty
Messenger RNA integumentary system chemical and pharmacologic phenomena hemic and immune systems Cell Biology Biology Biochemistry Molecular biology chemistry.chemical_compound Thymocyte Endocrinology chemistry Cell culture Cell surface receptor Internal medicine Ionomycin Gene expression medicine lipids (amino acids peptides and proteins) Receptor Protein kinase A Molecular Biology |
| Zdroj: | Journal of Biological Chemistry. 263:4043-4046 |
| ISSN: | 0021-9258 |
| DOI: | 10.1016/s0021-9258(18)68885-4 |
| Popis: | The phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) and the calcium ionophore ionomycin differentially regulated the expression of the gamma-T-cell antigen receptor (gamma-TCR) gene in human thymocytes. Ionomycin induced an increase in gamma-TCR mRNA while TPA prevented this effect. The effects of ionomycin and TPA are completely reversed upon their removal. Protein kinase C-depleted cells are unable to respond to TPA but the induction of gamma-TCR by ionomycin was unimpaired. The regulation of gamma-TCR gene expression by TPA and ionomycin occurred at the level of gene transcription, whereas the rate of gamma-TCR mRNA degradation was unaffected by TPA or ionomycin. |
| Databáze: | OpenAIRE |
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