| Autor: |
Jang-Ming Lee, Meng-Kun Tsai, Chun Jean Lee, Hao-Chih Tai, Rong Phong Hsieh, Robert J. Chen, Kimiyoshi Tsuji, Lee Yc, Chung-Yi Hu, Chung Nan Weng, Pei Wen Yang, Bor-Luen Chiang, Ching Fu Tu, Kun Hsiung Lee |
| Rok vydání: |
2002 |
| Předmět: |
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| Zdroj: |
Transplantation. 73:193-197 |
| ISSN: |
0041-1337 |
| DOI: |
10.1097/00007890-200201270-00007 |
| Popis: |
Background. In pig-to-human discordant xenotrans-plantation, the xenograft can be rejected by a formidable human xenogenic T-cell response, even if the graft has gone through hyperacute rejection or delayed xenograft rejection (acute vascular rejection). We therefore examined, in this study, whether the human-to-pig cellular response could be attenuated through the generation of a transgenic pig for human HLA II. Methods. With the technique of microinjection, we produced the HLA DPw0401 transgenic pig. The expression of the HLA DPw0401 gene on peripheral blood mononuclear cells (PBMCs) of the transgenic pig was examined by reverse transcriptase-polymerase chain reaction and flow cytometry. The antigenicity of the transgenic HLA DPw0401 molecule was tested by the HLA DPw0401-primed lymphocyte test reagent. The cellular response was analyzed by xenogenic mixed lymphocyte culture. Results. The mRNA and protein of HLA DPw0401 were expressed in the PBMCs of the transgenic pig. The PBMCs of the HLA transgenic pig induced a stronger cellular reaction to HLA DPw0401-primed lymphocyte test reagents than the nontransgenic littermate pig (n=7, P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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