A Facile Method for Preparing Temperature-Responsive Cell Culture Surfaces by Using a Thioxanthone Photoinitiator Immobilized on a Polystyrene Surface
| Autor: | Kazuhiro Fukumori, Yoshikatsu Akiyama, Masayuki Yamato, Teruo Okano |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Materials science
Nanostructure Renewable Energy Sustainability and the Environment fungi Polyacrylamide food and beverages Energy Engineering and Power Technology 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Thioxanthone 01 natural sciences 0104 chemical sciences Biomaterials chemistry.chemical_compound chemistry Chemical engineering Polymerization Polymer chemistry Materials Chemistry Poly(N-isopropylacrylamide) Polystyrene 0210 nano-technology Photoinitiator Visible spectrum |
| Zdroj: | ChemNanoMat. 2:454-460 |
| ISSN: | 2199-692X |
| DOI: | 10.1002/cnma.201600056 |
| Popis: | We developed a new facile method for the preparation of temperature-responsive cell culture surfaces (TRCS) by photo-polymerization of N-isopropylacrylamide using a surface-immobilized photoinitiator. In the first step, thioxanthone groups, which served as the photoinitiator, were directly formed on polystyrene culture dish surfaces (TX-PSt). Second, poly(N-isopropylacrylamide) (PIPAAm) was grafted onto the TX-PSt surfaces (PIPAAm-TX-PSt) by using the visible light or light-emitting diode irradiation-induced surface-initiated polymerization method. By optimizing the amount of grafted PIPAAm, the resulting PIPAAm-TX-PSt successfully exhibited characteristics of a TRCS. In addition, a striped micropatterned polyacrylamide area was successively formed on the photoirradiated PIPAAm-TX-PSt surface by using a chromium photomask. These results show that temperature-responsive cell culture surfaces can be prepared through this new method. |
| Databáze: | OpenAIRE |
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