Decursin induces apoptosis by regulating AMP-activated protein kinase and Bax/Bcl- 2 pathway in HepG2 cell line
| Autor: | Nada A. Alhamed, Dana Almohazey, Ebtesam A. Al-Suhaimi, Sarah Ameen Almofty, Meneerah A. Aljafary, Najla Alhamed, Adeeb Shehzad, Vijaya Ravinayagam, Noor A. Al-Rashid |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
biology medicine.diagnostic_test Cost effectiveness Chemistry AMPK 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Complementary and alternative medicine Western blot Apoptosis 030220 oncology & carcinogenesis Cancer research biology.protein medicine MTT assay Viability assay Propidium iodide Caspase |
| Zdroj: | European Journal of Integrative Medicine. 24:17-22 |
| ISSN: | 1876-3820 |
| Popis: | Introduction Hepatocellular carcinoma (HCC) is a lethal cancer. Phytomedicine may have benefits and advantages including the potential therapeutic efficacy, safety and cost effectiveness. Decursin, extracted from Angelica gigasNakai, is used in South Korea and China for treating diseases including cancer. The aim of this study is to evaluate the apoptotic effect of Decursin on the HCC cell line (HepG2). Methods The apoptotic effect of Decursin was evaluated through dose and time dependent manner using HepG2 cell line. The cell viability was analyzed by MTT assay using Decursin (10, 20, 30, 40 and 50 μM, for 24h). For the optimal concentration, the following doses were studied: 10, 20 and 50 μM to 24h for dose fixation using western blot technique (p-AMPK, t-AMPK, p-ACC, t-ACC). 20 μM dose was appropriate to evaluate the protein expression in 24h. The time dependent effect of 20 μM Decursin was evaluated at 4h, 8h, 12h and 24h using western blot technique (p-AMPK, t-AMPK, p-ACC, t-ACC). Results Compared with control cells, this time dependent study showed effective upregulation of the activated AMPK pathway at 24h. The apoptotic effect of Decursin was construed using propidium iodide staining for cell cycle analysis. The apoptotic and antiproliferative effect of Decursin were elucidated by protein expression of pro-caspase 3, Bcl-2, and Bax at the concentration of 20 μM dose for 6h, 12h, and 24h. Conclusion Our results confirm that Decursin-induced apoptosis is mediated through inhibition of anti-apoptotic proteins Bcl-2 and activation of caspase cascade by regulating the expression of AMPK, ACC, and Bax. Decursin might be a potential therapeutic candidate for the treatment of HCC. |
| Databáze: | OpenAIRE |
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