| Autor: |
Govindarajan Rathi Bhuvaneswari, Kurcheti Pani Prasad, Sudhagar Arun Sudhagar, Marrappan Makesh, Kezhedath Jeena |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Aquaculture Research. 46:1417-1425 |
| ISSN: |
1355-557X |
| DOI: |
10.1111/are.12295 |
| Popis: |
Pangasius (Pangasianodon hypophthalmus) is a commercially important candidate species in freshwater aquaculture and it is important to understand the immune system of pangasius against infectious disease. The present study was aimed at the purification, characterization and quantification of serum IgM in pangasius (P. hypophthalmus). Serum IgM was purified by diethylaminoethyl (DEAE) cellulose based ion exchange chromatography. The molecular weight of native immunoglobulin was found to be 798 kDa. Heavy (H) and light chains were found to possess molecular weight of 70.1 and 26 kDa respectively. A 248 bp segment of IgM H chain gene of pangasius was amplified and sequenced (partial). The antisera raised against pangasius immunoglobulin cross-reacted with the immunoglobulin H chain of catfish such as Clarias gariepinus and Clarias batrachus, but not with their light chain indicating epitope sharing among IgM H chains in these catfish. The produced polyclonal antisera were used to develop an enzyme linked immuosorbent assay to quantify IgM levels in pangasius. In conclusion, the present study provides its future implications for epidemiology and immunology studies in pangasius. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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