Self-association of daunomycin antibiotic in various buffer solutions

Autor:	V. V. Khomich, David B. Davies, Maxim P. Evstigneev
Rok vydání:	2006
Předmět:	HEPES Tris chemistry.chemical_compound chemistry Dimer Chemical shift Analytical chemistry Physical chemistry Physical and Theoretical Chemistry Chromophore Antiparallel (biochemistry) Two-dimensional nuclear magnetic resonance spectroscopy Buffer (optical fiber)
Zdroj:	Russian Journal of Physical Chemistry. 80:741-746
ISSN:	1531-863X 0036-0244
DOI:	10.1134/s003602440605013x
Popis:	The self-association of an antitumor antibiotic, daunomycin (DAU), in various buffer solutions, including water-salt solutions, phosphate buffer, HEPES, and TRIS, was studied by 1D and 2D 1H NMR spectroscopy at 500 MHz. The two-dimensional NOESY spectra and the concentration and temperature dependences of the chemical shifts of antibiotic protons in the solvents studied showed that the self-association parameters of DAU were independent of the type of buffer solutions. The most probable two spatial structures of the dimer of DAU in solution were constructed by the method of molecular mechanics. The mutual orientation of the chromophores was parallel in one structure and antiparallel in the other.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a345e312d8d3da2ae34b7dd98fce8330 https://doi.org/10.1134/s003602440605013x Zobrazit plný text záznamu Full text from SpringerLink