| Autor: |
John G. McCarron, Christopher D. Saunter, Calum Wilson, John M. Girkin, Penelope F. Lawton |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII. |
| DOI: |
10.1117/12.2506993 |
| Popis: |
The vascular endothelium is a complex single layered network of cells which signal via the release of Ca2+ ions; the study of endothelial cell function and interactions in response to stimuli provides useful information for medical research into, for example, hypertension, diabetes and heart failure. A side viewing GRIN imaging system has previously been used to view calcium signalling in the endothelium [1] utilising a low numerical aperture GRIN rod and microscope objective to increase the imaging depth and image a large number of cells over the curved inner artery surface. This allows cells to be imaged in near-physiological conditions, as opposed to imaging of flattened arteries; however, the use of GRIN lenses introduces optical aberrations. Resolution is also limited from using a low numerical aperture system. In this work we investigate this important imaging challenge further with a view to compensating for both the cylindrically-curved geometry of the arteries and field-aberrations present in the optical system. The field aberrations in the imaging optics and resulting from the curved surface/planar sensor mismatch are quantified to allow for corrections to be made through introducing field curvature and aberration correction into the imaging path. This new instrumentation opens up the potential to image calcium signalling within large numbers of cells to try and understand the complex patterns which are produced in response to a range of stimuli. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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