Surprisingly Effective Priming of CD8 + T Cells by Heat-Inactivated Vaccinia Virus Virions
| Autor: | Stewart A Smith, Inge E. A. Flesch, Sarah N Croft, Yik Chun Wong, David C. Tscharke |
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| Rok vydání: | 2020 |
| Předmět: |
Inactivated Vaccinia Virus
0303 health sciences viruses T cell Immunology Priming (immunology) Biology Microbiology Virology Virus 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine.anatomical_structure Viral replication chemistry Insect Science Inactivated vaccine medicine Cytotoxic T cell Vaccinia 030304 developmental biology 030215 immunology |
| Zdroj: | Journal of Virology. 94 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Robust priming of CD8+ T cells by viruses is considered to require infection and de novo expression of viral antigens. A corollary of this is that inactivated viruses are thought of as being inevitably poor vaccines for eliciting these responses. In contrast to this dogma, we found that some antigens present in vaccinia virus (VACV) virions prime strong CD8+ T cell responses when the virus was rendered noninfectious by heat. More surprisingly, in some cases these responses were similar in magnitude to those primed by infectious virus administered at an equivalent dose. Next, we tested whether this was a special property of particular antigens and their epitopes and found that foreign epitopes tagged onto three different VACV virion proteins were able to elicit CD8+ T cell responses irrespective of whether the virus was viable or heat killed. Further, the polyfunctionality and cytotoxic ability of the CD8+ T cells primed by these VACVs was equivalent irrespective of whether they were administered to mice as inactivated or live viruses. Finally, we used these VACVs in prime-boost combinations of inactivated and live virus and found that priming with dead virus before a live booster was the most immunogenic regime. We conclude that VACV virions can be efficient vectors for targeting antigens to dendritic cells for effective priming of CD8+ T cells, even when rendered noninfectious and speculate that this might also be the case for other viruses.IMPORTANCE The design of viral vectored vaccines is often considered to require a trade-off between efficacy and safety. This is especially the case for vaccines that aim to induce killer (CD8+) T cells, where there is a well-established dogma that links infection in vaccinated individuals with effective induction of immunity. However, we found that some proteins of vaccinia virus generate strong CD8+ T cell responses even when the virus preparation was inactivated by heat prior to administration as a vaccine. We took advantage of this finding by engineering a new vaccine vector virus that could be used as an inactivated vaccine. These results suggest that vaccinia virus may be a more versatile vaccine vector than previously appreciated and that in some instances safety can be prioritized by the complete elimination of viral replication without a proportional loss of immunogenicity. |
| Databáze: | OpenAIRE |
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