Baciloscopia para Tuberculose pulmonar. Estudo multicêntrico do esfregaço para baciloscopia de escarro no diagnóstico da tuberculose pulmonar segundo a Organização Mundial da Saúde e o Ministério da Saúde

Autor: Cacilda Rosa Cardoso da Silva, Leonilda Chiari Galle, Suely Yoko Mizuka Ueki, Andréa Gobetti Vieira Coelho, Maria Izabel Pereira Ferreira, Dalva Cristina Girello Aily, Andréa Rezende Leite, Maria do Rosário Assad Goloni, Maria de Lourdes M. Shikama, Heloisa da Silveira Paro Pedro, Regina Ruivo Ferro e Silva, Susilene Maria Tonelli Nardi
Rok vydání: 2011
Předmět:
Zdroj: Revista de Patologia Tropical. 39
ISSN: 1980-8178
0301-0406
DOI: 10.5216/rpt.v39i4.13062
Popis: In the diagnosis of pulmonary tuberculosis, sputum bacilloscopy was standardized by the WHO for slide preparation, staining, and reading the smear. The Brazilian Ministry of Health adopted this standardization for staining and reading, but the smear was extended to 2/3 of the slide, different to the elliptical format recommended by the WHO. This study aimed at comparing the two smear techniques by evaluating the sensitivity and specificity, preparation and reading times, and biosafety issues. Eight regional laboratories of the Adolfo Lutz Institute participated. Over three months, slides were prepared for 356 sputum samples using the two techniques. Reading of slides employed semi-quantitative counts using bacilloscopy index scale as recommended by the WHO and Brazilian Ministry of Health. The sensitivity of the elliptical smear was 97.0%, specificity 97.5%, accuracy 97.2%, and error 2.8%. Agreement of classification according to the scale was: 80.7% for rare BAAR, 83.7% for 1+, 89.8% for 2+, 90.9% for 3+ and 97.5% for negative cases (Kappa=0.87 p
Databáze: OpenAIRE