Effect of aging on EGF-stimulated replication of specific genes in rat hepatocytes

Autor: Vilhelm A. Bohr, George S. Roth, Shoichi Kitano, Cynthia M. Haggerty, Tanya D. Reed, Alfred May
Rok vydání: 1998
Předmět:
Zdroj: Journal of Cellular Physiology. 176:32-39
ISSN: 1097-4652
0021-9541
DOI: 10.1002/(sici)1097-4652(199807)176:1<32::aid-jcp4>3.0.co;2-9
Popis: EGF-stimulated replication of specific genes was examined in primary hepatocyte cultures from mature (6 months) and senescent (24 months) rats. Basal and EGF-stimulated [3H]thymidine incorporation and DNA polymerase α activities, as well as total cellular DNA, were also assessed. The genes examined were dihydrofolate reductase (DHFR) and c-myc, as well as total mitochondrial DNA (mt DNA). Although [3H]thymidine incorporation, DNA polymerase α activity, total cellular DNA, DHFR, and c-myc gene specific DNA replication stimulated by EGF are reduced with age, mt DNA replication is not affected by either EGF or age. Chromosomal DNA replication is mediated mainly by DNA polymerase α while mt DNA replication is mediated by its own DNA polymerase γ. Thus, the age-related decline in stimulated DNA replication appears to be associated mainly with the DNA polymerase α activation pathway. J. Cell. Physiol. 176:32–39, 1998. Published 1998 Wiley-Liss, Inc.1
Databáze: OpenAIRE
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