Expression of Integrin αvβ6 and TGF-β in Scarless vs Scar-forming Wound Healing
| Autor: | Humphrey Gardner, Dariush Honardoust, David A. Hart, Lari Häkkinen, Hannu Larjava, Corrie L. Gallant-Behm, Ameneh Eslami, Colin Wiebe |
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| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 57:543-557 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1369/jhc.2009.952572 |
| Popis: | Oral mucosal wounds heal with reduced scar formation compared with skin. The epithelial integrin αvβ6 is induced during wound healing, and it can activate fibrogenic transforming growth factor β1 (TGF-β1) and anti-fibrogenic TGF-β3 that play key roles in scar formation. In this study, expression of β6 integrin and members of the TGF-β pathway were studied in experimental wounds of human gingiva and both gingiva and skin of red Duroc pigs using real-time PCR, gene microarrays, and immunostaining. Similar to human wounds, the expression of β6 integrin was induced in the pig wounds 7 days after wounding and remained upregulated >49 days. The αvβ6 integrin was colocalized with both TGF-β isoforms in the wound epithelium. Significantly higher expression levels of β6 integrin and TGF-β1 were observed in the pig gingival wounds compared with skin. Early gingival wounds also expressed higher levels of TGF-β3 compared with skin. The spatio-temporal colocalization of αvβ6 integrin with TGF-β1 and TGF-β3 in the wound epithelium suggests that αvβ6 integrin may activate both isoforms during wound healing. Prolonged expression of αvβ6 integrin along with TGF-β3 in the gingival wound epithelium may be important in protection of gingiva from scar formation. (J Histochem Cytochem 57:543–557, 2009) |
| Databáze: | OpenAIRE |
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