Identification of soyasaponins by liquid chromatography–thermospray mass spectrometry

Autor:	R Pace, Gianluca Papeo, N Fuzzati, F Peterlongo
Rok vydání:	1997
Předmět:	Chemical ionization Chromatography Chemistry Organic Chemistry Thermospray General Medicine Reversed-phase chromatography Fast atom bombardment Mass spectrometry Biochemistry High-performance liquid chromatography Analytical Chemistry Column chromatography Mass spectrum
Zdroj:	Journal of Chromatography A. 777:233-238
ISSN:	0021-9673
DOI:	10.1016/s0021-9673(97)00157-x
Popis:	Saponins are important bioactive molecules widespread in the plant kingdom. Soyasaponins, isolated from Glicine max (Leguminosae), have been shown to exhibit various biological activities, e.g., an inhibitory effect on lipid-oxidation and liver-lesion generation and an improving effect on hyper-cholesteremia. Mass spectral investigation of these metabolites requires soft ionization techniques such as desorption chemical ionization, fast atom bombardment and thermospray mass spectrometry. A high-performance liquid chromatographic method was developed for thermospray mass spectrometric analysis of saponins contained in soybean flour extracts. The analyses were performed using a ternary eluent (water–acetonitrile–methanol) in gradient conditions with post-column addition of aqueous ammonium acetate. Six saponin components could be separated and identified. The mass spectra obtained provided information concerning both molecular masses and aglycone composition.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a2fbc106a3426ac4301e8facd9c1daa8 https://doi.org/10.1016/s0021-9673(97)00157-x Zobrazit plný text záznamu Full Text from ScienceDirect