Ferrocytochrome c and deoxyhemoglobin in the reaction with the iron cysteamine nitrosyl complex {Fe2[S(CH2)2NH3]2(NO)4}SO4·2.5H2O

Autor: B. L. Psikha, N. I. Shkondina, Natalia A. Sanina, T. N. Rudneva, S. M. Aldoshin, Alexander I. Kotelnikov, L. A. Syrtsova
Rok vydání: 2010
Předmět:
Zdroj: Russian Chemical Bulletin. 59:1994-1998
ISSN: 1573-9171
1066-5285
Popis: By an example of the iron cysteamine nitrosyl complex {Fe2[S(CH2)2NH3]2(NO)4}SO4··2.5H2O (CAC), it was shown for the first time that the hydrolysis of this NO donor in the presence of ferrocytochrome c (cyt c2+) affords the iron nitrosyl complex NO-cyt c2+, which serves as the NO depot. The rate constant of NO release from CAC was determined from the kinetics of the formation of NO-cyt c2+. At pH 3.0 the rate constant is (2.7±0.1)·10−3 s−1. Ferrocytochrome c produces a less stabilizing effect on CAC than deoxyhemoglobin (Hb). Thus in the presence of cyt c2+, the reaction is completed in 1 h, whereas NO is released from a solution of CAC (2·10−4 mol L−1) in the presence of Hb during 40 h. The previously unknown stabilization of iron nitrosyl complexes by hemoglobin was found.
Databáze: OpenAIRE
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