The steady state current at a microdisk biosensor

Autor:	Chatuporn Phanthong, Mithran Somasundrum
Rok vydání:	2003
Předmět:	Immobilized enzyme biology Chemistry General Chemical Engineering Enzyme electrode Analytical chemistry Amperometry Analytical Chemistry Ion selective electrode Electrode Electrochemistry biology.protein Glucose oxidase Steady state (chemistry) Biosensor
Zdroj:	Journal of Electroanalytical Chemistry. 558:1-8
ISSN:	1572-6657
DOI:	10.1016/s0022-0728(03)00370-x
Popis:	We have modeled the steady state current generated at a microdisk enzyme electrode where a reaction product of the enzyme reacts at the electrode itself. Simple approximate expressions have been derived for the response in limiting cases, as well as a semi-empirical expression suitable for all cases. The model was tested using Pt microdisks modified by films of poly(vinyl alcohol) containing glucose oxidase. From the variation of the current with film thickness, the diffusion coefficient of glucose in the film was estimated, as well as the value of kcat/KM for the immobilized enzyme.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a2d92ca3651ad8066b9db8981b0605ca https://doi.org/10.1016/s0022-0728(03)00370-x Zobrazit plný text záznamu Full Text from ScienceDirect