Autor: Paul S. Gross, Gregory W. Warr, David J. McKillen, Yian Ann Chen, Matthew J. Jenny, Shuyuan Wu, Jonas S. Almeida, R. W. Chapman
Rok vydání: 2004
Předmět:
Zdroj: BMC Bioinformatics. 5:191
ISSN: 1471-2105
DOI: 10.1186/1471-2105-5-191
Popis: Background Expression microarrays are increasingly used to characterize environmental responses and host-parasite interactions for many different organisms. Probe selection for cDNA microarrays using expressed sequence tags (ESTs) is challenging due to high sequence redundancy and potential cross-hybridization between paralogous genes. In organisms with limited genomic information, like marine organisms, this challenge is even greater due to annotation uncertainty. No general tool is available for cDNA microarray probe selection for these organisms. Therefore, the goal of the design procedure described here is to select a subset of ESTs that will minimize sequence redundancy and characterize potential cross-hybridization while providing functionally representative probes.
Databáze: OpenAIRE
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