Simultaneous analysis of fructose and creatinine in urine samples using GC–MS/MS and enzymatic methods
| Autor: | Peter Kotora, Václav Matěj Bierhanzl, Radomír Čabala, Alexandra Hengerics Szabó, Viktória Ferenczy, Jaroslav Blaško, D. Behulova, Róbert Kubinec, Ngô Mạnh Thắng |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Analyte Creatinine Chromatography Silylation Chemistry 010401 analytical chemistry General Chemistry Urine Mass spectrometry 01 natural sciences 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Gas chromatography Gas chromatography–mass spectrometry Derivatization |
| Zdroj: | Monatshefte für Chemie - Chemical Monthly. 147:1343-1347 |
| ISSN: | 1434-4475 0026-9247 |
| Popis: | This work focuses on the simultaneous analysis of creatinine and fructose with the gas chromatographic tandem mass spectrometric (GC–MS/MS) method after silylation with hexamethyldisilazane and N,O-bis(trimethylsilyl)trifluoroacetamide and the comparison of GC–MS/MS and enzyme-catalyzed photometric analysis of creatinine. The GC–MS/MS method allows the direct silylation of urine samples without sample pretreatment before derivatization. Due to this option, the analysis and diagnostics of inborn metabolic diseases are faster than in the case of a separate determination of both analytes. |
| Databáze: | OpenAIRE |
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