Large-scale preparation of a nitrile-hydrolysing biocatalyst: Rhodococcus R 312 (CBS 717.73)

Autor:	Kurt Faber, C. Jarret, Andrew Willetts, Wolfgang Kroutil, Ingrid Osprian, Romano V. A. Orru, Ulfried Felfer, Ulrike T. Strauss
Rok vydání:	1999
Předmět:	biology Nitrile Chemistry Process Chemistry and Technology fungi food and beverages Bioengineering biology.organism_classification Biochemistry Nitrilase Catalysis Hydrolysis chemistry.chemical_compound Biotransformation Nitrile hydratase Biocatalysis Organic chemistry Fermentation Rhodococcus
Zdroj:	Journal of Molecular Catalysis B: Enzymatic. 6:555-560
ISSN:	1381-1177
DOI:	10.1016/s1381-1177(99)00009-0
Popis:	Lyophilized cells of Rhodococcus R 312 (CBS 717.73) can be employed as an easy-to-use biocatalyst for the biocatalytic hydrolysis of nitriles to furnish the corresponding carboxamides or acids on a preparative scale. The following practical aspects are advantageous: (i) Fermentation yields a high biomass (∼10 g dry cell weight l−1), (ii) enzyme induction is not required, (iii) a maximum of activity is obtained at the late exponential growth phase (25.7 μmol min−1 mg−1), which can be monitored by a simple photometic assay and (iv) the cells can be stored at +4°C for several months without significant loss of activity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a2b35f3ac1b7b5be9c9f5d03a8c10dce https://doi.org/10.1016/s1381-1177(99)00009-0 Zobrazit plný text záznamu Full Text from ScienceDirect