ESR and Optical Studies on the Interaction between Cu(II) and Pepsin

Autor:	Wolfgang Lohmann, Harald Neubacher, Luigi Sportelli
Rok vydání:	1978
Předmět:	Conformational change Crystallography Aqueous solution Pepsin biology Molar ratio Chemistry law biology.protein Spectroscopy Electron paramagnetic resonance General Biochemistry Genetics and Molecular Biology law.invention
Zdroj:	Zeitschrift für Naturforschung C. 33:321-325
ISSN:	1865-7125 0939-5075
DOI:	10.1515/znc-1978-5-603
Popis:	The interaction of Cu (II) with the protein pepsin has been investigated by means of electron spin resonance (ESR) and optical spectroscopy. Depending on the molar ratio of Cu(II) and pepsin in aqueous solution two different complexes are formed. A third complex can be detected after a reaction time of several days, attributed to a complex with a conformationally changed pepsin. The presence of inhibitors 1,2-epoxy-3-phenoxypropane (EPP) or diazoacetyl-ethylester (DAE) seems to hinder the conformational change. The structure of the three complexes is discussed.
Databáze:	OpenAIRE
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