Evidence for T Lymphocyte Subpopulations in the Rabbit
| Autor: | Michael W. Fanger, Andy C. Reese, Melvin D. Schoenberg, Abram B. Stavitsky, Alice L. Reese |
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| Rok vydání: | 1974 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 112:1971-1980 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.112.6.1971 |
| Popis: | A goat antibody to rabbit thymus cells (ATG) was used to study T lymphocyte distributions and subpopulations in the rabbit. ATG inhibited transformation of peripheral blood lymphocytes stimulated by phytohemagglutinin (PHA) and concanavalin A (Con A) but not by goat anti-rabbit Fab. The distribution of T lymphocytes was determined by the ability of lymphocytes from the various tissues to absorb this inhibitory activity. Lymph node, peripheral blood, and thymus lymphocytes, in that order, were most capable of absorbing the ATG activity. Fluorescein-conjugated ATG caused fluorescence of 100% of thymus cells but lower percentages of lymphocytes from other organs. In addition, the intensity of staining by fluorescent antibody varied among lymphocytes even from the same organ. The results indicate the presence of rabbit T cell subpopulations which differ in the quantity of T antigen per cell. The effect of ATG on mitogen stimulation of lymphocytes from the various lymphoid organs indicated that the T cell subsets differed in their response to PHA and Con A, and in their susceptibility to the inhibitory effect of ATG. Evidence is also presented which suggests the presence of lymphocytes which possess both surface T antigen and Ig determinants. |
| Databáze: | OpenAIRE |
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